更新時(shí)間:2016-05-10
我司BRL大鼠肝細(xì)胞,產(chǎn)地美國(guó)ATCC細(xì)胞庫(kù),所有出入庫(kù)細(xì)胞均經(jīng)過(guò)嚴(yán)格的細(xì)胞質(zhì)量檢測(cè),確保細(xì)胞無(wú)污染,符合檢測(cè)合格標(biāo)準(zhǔn)。訂購(gòu)7~15 天內(nèi)即可發(fā)貨,采用快遞郵寄,確保細(xì)胞Z短時(shí)間內(nèi)送到客戶(hù)手中。
免費(fèi)咨詢(xún):021-54720761
BRL大鼠肝細(xì)胞培養(yǎng)注意事項(xiàng):
1、實(shí)驗(yàn)進(jìn)行前,無(wú)菌室及無(wú)菌操作臺(tái)(laminarflow)以紫外燈照射30-60分鐘滅菌,以70%ethanol擦拭無(wú)菌操作抬面,并開(kāi)啟無(wú)菌操作臺(tái)風(fēng)扇運(yùn)轉(zhuǎn)10分鐘后,才開(kāi)始實(shí)驗(yàn)操作。
2、BRL大鼠肝細(xì)胞操作工作區(qū)域應(yīng)保持清潔及寬敞,必要物品,例如試管架、吸管吸取器或吸管盒等可以暫時(shí)放置,其它實(shí)驗(yàn)用品用完即應(yīng)移出,以利于氣流之流通。實(shí)驗(yàn)用品以70%ethanol擦拭后才帶入無(wú)菌操作臺(tái)內(nèi)。
3、小心取用無(wú)菌之實(shí)驗(yàn)物品,避免造成污染。勿碰觸吸管尖頭部或是容器瓶口,亦不要在打開(kāi)之容器正上方操作實(shí)驗(yàn)。容器打開(kāi)后,以手夾住瓶蓋并握住瓶身,傾斜約45°角取用,盡量勿將瓶蓋蓋口朝上放置桌面。
3、工作人員應(yīng)注意自身之安全,須穿戴實(shí)驗(yàn)衣及手套后才進(jìn)行實(shí)驗(yàn)。
目錄號(hào) | CC-Y3004 |
細(xì)胞英文(簡(jiǎn)稱(chēng)) | BRL |
細(xì)胞名稱(chēng) | |
背景資料 | 收到細(xì)胞,請(qǐng)查看瓶子是否有破裂,培養(yǎng)基是否漏出,是否渾濁,如有請(qǐng)盡快和我們。如包裝完好,取出25cm2培養(yǎng)瓶,75%酒精消毒,拆下封口膜,在顯微鏡下觀察細(xì)胞形態(tài)是否正常,細(xì)胞的貼壁情況以及細(xì)胞密度,然后放入37℃,5%CO2細(xì)胞培養(yǎng)箱中靜置2-3小時(shí),以穩(wěn)定細(xì)胞狀態(tài)。 |
細(xì)胞來(lái)源 | ATCC |
代次 | P3 |
規(guī)格 | T25 |
細(xì)胞數(shù) | 50-100萬(wàn) |
價(jià)格 | 電議 |
生物安全級(jí)別 | 2 |
組織來(lái)源 | 肝 |
細(xì)胞形態(tài) | 貼壁;上皮細(xì)胞樣 |
細(xì)胞活力 | 95%(Viability by Trypan Blue Exclusion) |
細(xì)胞檢測(cè) | 細(xì)胞不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌 |
培養(yǎng)條件 | RPMI-1640 +10% FBS;37℃,5% CO2 |
傳代方法 | 建議1:2-1:3 兩天換液一次 |
凍存條件 | *培養(yǎng)基50%+40%FBS+10%DMSO |
提供的細(xì)胞類(lèi)型有細(xì)胞系,每個(gè)細(xì)胞發(fā)貨前都必須經(jīng)過(guò)實(shí)驗(yàn)室QC檢測(cè),確保每個(gè)批次的質(zhì)量。是上海生物行業(yè)*的代理供應(yīng)商,提供報(bào)價(jià)、咨詢(xún)及技術(shù)服務(wù),選購(gòu)。
我司其他相關(guān)銷(xiāo)售產(chǎn)品:
Cs-(019)-001887 SK-HEP-1細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001888 NCI-H838[H838]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001889 SK-OV-3[SKOV-3]細(xì)胞株/細(xì)胞,人卵巢癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001890 HPAC細(xì)胞株/細(xì)胞,人胰腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001891 PC-3細(xì)胞株/細(xì)胞,人前列腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001892 SW 1088[SW-1088;SW1088]細(xì)胞株/細(xì)胞,人腦星型膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001893 EB-3[EB3]細(xì)胞株/細(xì)胞,人淋巴樣Burkitt淋巴瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001894 HT-3細(xì)胞株/細(xì)胞,人子宮頸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001895 TT細(xì)胞株/細(xì)胞,人骨髓樣甲狀腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001896 ES-2細(xì)胞株/細(xì)胞,人卵巢透明細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001897 LoVo細(xì)胞株/細(xì)胞,人結(jié)腸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001898 HT-29細(xì)胞株/細(xì)胞,人結(jié)腸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001899 TE 115.T細(xì)胞株/細(xì)胞,人軟組織纖維瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001900 Cates-1B細(xì)胞株/細(xì)胞,人睪丸淋巴胚胎性癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001901 SNU-398細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001902 Hep 3B2.1-7[Hep 3B;Hep-3B;Hep3B]細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001903 SNU-182細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001904 PLC/PRF/5細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001905 WERI-Rb-1細(xì)胞株/細(xì)胞,人視網(wǎng)膜母細(xì)胞株/細(xì)胞瘤 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001906 PA-1細(xì)胞株/細(xì)胞,人卵巢畸胎瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001907 Hs 578T細(xì)胞株/細(xì)胞,人乳腺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001908 Hs 578Bst細(xì)胞株/細(xì)胞,人乳腺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001909 TE 354.T細(xì)胞株/細(xì)胞,人皮膚基底細(xì)胞株/細(xì)胞癌 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001910 TE 353.Sk細(xì)胞株/細(xì)胞,人正常皮膚細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001911 SW480[SW-480]細(xì)胞株/細(xì)胞,人結(jié)腸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001912 SW620[SW-620]細(xì)胞株/細(xì)胞,人結(jié)腸腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001913 NCI-H1299細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001914 PC-12細(xì)胞株/細(xì)胞,大鼠腎上腺嗜鉻細(xì)胞株/細(xì)胞瘤 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001915 COS-7細(xì)胞株/細(xì)胞,非洲青猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001916 TOV-112D細(xì)胞株/細(xì)胞,人上皮性卵巢癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001917 MRC-5細(xì)胞株/細(xì)胞,人正常胚肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001918 NCI-H1563[H1563]細(xì)胞株/細(xì)胞,人胚肺細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001919 NCI-H2170[H2170]細(xì)胞株/細(xì)胞,人肺鱗狀細(xì)胞株/細(xì)胞癌 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001920 Hs888Lu細(xì)胞株/細(xì)胞,人肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001921 SW 1271[SW-1271;SW1271]細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001922 Hep G2細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001923 SJCRH30[RC13;RMS 13;SJRH30]細(xì)胞株/細(xì)胞,人骨髓橫紋肌肉癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001924 C2C12細(xì)胞株/細(xì)胞,人肌肉成纖維細(xì)胞株/細(xì)胞瘤 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001925 G-7細(xì)胞株/細(xì)胞,小鼠骨骼肌肌肉母瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001926 Sol8細(xì)胞株/細(xì)胞,小鼠骨骼肌肌肉母細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001927 BLO-11細(xì)胞株/細(xì)胞,小鼠骨骼成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001928 NOR-10細(xì)胞株/細(xì)胞,小鼠骨骼肌成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001929 NCI-H596[H596]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001930 NCI-H520[H520]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001931 NCI-H1703[H1703]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001932 NCI-H226[H226]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001933 SK-MES-1細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001934 NCI-H1869[H1869]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001935 SW 900[SW-900;SW900]細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001936 7F2細(xì)胞株/細(xì)胞,小鼠雜交瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001937 3T3-L1細(xì)胞株/細(xì)胞,小鼠脂肪細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001938 THLE-3細(xì)胞株/細(xì)胞,人肝上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001939 PT67細(xì)胞株/細(xì)胞,小鼠逆轉(zhuǎn)錄病毒包裝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001940 hFOB 1.19細(xì)胞株/細(xì)胞,人SV40轉(zhuǎn)染成骨細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-001941 HIT-T15細(xì)胞株/細(xì)胞,倉(cāng)鼠beta胰島細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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